Figure 5.

Continuous, real-time molecular monitoring in a flowing sample stream. We challenged a melamine-detecting sensor in undiluted, flowing whole milk to demonstrate the real-time measurement of this target. Specifically, we fabricated these sensors with a T20 construct and challenged them against varying pulses of the target in flowing whole milk over the course of several hours. As expected, our sensors responded correspondingly and rapidly to the spiked concentrations (300 and 600 μM, respectively), and the signal returned back to the baseline (within 2%) when the sensor is returned to melamine-free milk.