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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Ann Neurol. 2016 Oct;80(4):600–615. doi: 10.1002/ana.24761

FIGURE 3:

FIGURE 3:

Expression of ATXN2-AS in postmortem spinocerebellar ataxia type 2 (SCA2) cerebella. (A, B) Validation of quantitative polymerase chain reaction (qPCR) primers for ATXN2 and ATXN2-AS by strand-specific reverse transcription PCR (SS-RT-PCR). SS-RT-PCRs suggest that exon 12 (for ATXN2, A) and exon 1/intron 1 junction (for ATXN2-AS, B) regions are unidirectionally transcribed. Each experiment was repeated 3 times; representative gel images are shown. (C, D) Expression level of ATXN2 (C) and ATXN2-AS (D) as determined by qPCR and normalized to ACTB in control (Ctl) and SCA2 human cerebella. Three control and 5 SCA2 brains were included. The ATXN2/ACTB or ATXN2-AS/ACTB ratio in control was normalized to 1. (E) Expression level of ATXN2-AS relative to ATXN2 in human control and SCA2 cerebella. The ATXN2/ACTB ratio in control was normalized to 100. Data are expressed as mean ± standard deviation for control (n = 3) and SCA2 (n = 5) brains. n.s. = not significant by Mann–Whitney test; −RT = no reverse transcription.