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(A,B), confocal-FLIM image and corresponding time-resolved confocal image of 40 nm fluorescent microspheres, as well as their enlarged images of the white marking areas. (C,D) STED-FLIM image and corresponding time-resolved STED image of 40 nm fluorescent microspheres, as well as their enlarged images of the white marking areas. Pixel dwell time: 4 μs; the exposure time per pixel: 45 μs; pixel size: 52 nm. STED, stimulated emission depletion
