FIG. 3.

Inhibition of CRISPR-SpCas9 catalytic activity in vitro with nucleic acid-based inhibitors. (A) Inhibition of in vitro SpCas9 cleavage activity over time. Inhibitor and crRNA (crE2) concentrations were set at 1 μM. Error bars are standard error of the mean (SEM). (B) Inhibition of 10 min in vitro cleavage assay reactions containing 0.25 μM tracrRNA and 0.3 μM crRNA (crEGIP). Error bars are standard error of the mean (SEM). (C) Inhibition of the same reaction, but with 0.25 μM sgRNA (sgEGIP). Error bars are standard error of the mean (SEM).