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. 2019 May 31;9:172. doi: 10.3389/fcimb.2019.00172

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Copyright © 2019 Mir and Balamurugan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Expression of PemK_Sa toxin protein. Lane 1 contains E. coli DE3 total cell lysate protein, Lanes 2, 4, and 6 contains uninduced total cell lysate protein of E. coli DE3. Lanes 3, 5, and 7 contains IPTG induced total cell lysate protein of E. coli DE3. Concentration: Lanes 1, 2, and 3 (50 μg) Lanes 4 and 5 (75 μg) and Lanes 6 and 7 (100 μg), respectively. (B) 100 kDa protein marker (C) Molecular weight corresponding to PemK_Sa is ~16 kDa. PemK_Sa protein was purified affinity binding analyzed by 12% SDS-PAGE. Lane 2, 3, and 4 contains purified elute fractions of PemK_Sa protein.