FIGURE 5.

Assay of the sensitivity of the strains to DNA damaging agents. The strains were cultivated until OD₆₀₀∼0.2 and serially diluted by 10 times. An aliquot of 10 μl for each diluted culture was spotted on the plates and treated with UV (15 and 25 J/m², in B), or on plates containing methyl methanesulfonate (MMS, 3 and 4 mM, in A), hydroxyurea (HU, 2 and 3 mM, in A) or cisplatin (10 and 15 μg/ml, in B). The plates were incubated at 75°C for 7–10 days. The experiments were performed for 2–3 times. Representative figures are shown.