Skip to main content
. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Radiat Res. 2017 Jun 14;188(2):191–203. doi: 10.1667/RR14667.1

FIG. 4.

FIG. 4.

Protein analyses at 1 month postirradiation and 7 days after MI. Representative Western blot images of heart tissue homogenates from nonirradiated control mouse hearts and hearts of mice that were whole-body irradiated using different sequences at 1 month postirradiation in a radiation and aging plus MI model. Images represent scans of radiograms for phosphorylated (p), total (T) and loading control on day 7 for the following proteins: VEGF-A (38 kDa) and GAPDH (panel A); p-Erk1/2, T-Erk1/2 (42/44 kDa) and GAPDH (panel C); p-Akt (Ser473), T-Akt (62 kDa) and GAPDH (panel E). Quantification and graphic representation of total protein levels and phosphorylation using densitometric analysis of phospho-band intensities after adjusting for corresponding GAPDH (36 kDa) and total band intensities of heart tissue homogenates at 1 month postirradiation in a radiation and aging plus MI model for the following proteins: VEGF-A, p-Erk1/2 and p-Akt (Ser473) (panels B, D and F, respectively). Group 1: nonirradiated control (solid black bars); group 2: 1H × 3 irradiation (dotted bars); group 3: 1H × 3 + 56Fe irradiation (diagonal line bars); and group 4: 56Fe + 1H × 3 irradiation (solid red bars). Results in all graphs are depicted as mean ± SEM (n = 6–8 animals per time point/group). Statistical significance was assigned when P < 0.05.