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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Cell Rep. 2019 Jan 2;26(1):54–64.e6. doi: 10.1016/j.celrep.2018.12.018

Figure 4. Local or Systemic EBIO Treatment Reduces Non-convulsive Seizure Frequency in DS Mice.

Figure 4.

(A) Diagram of targeting of the thalamus with guide cannulas and tungsten electrodes for unit recordings. Inset: depth electrodes were positioned in nRT.

(B) Quantification of effects of intra-nRT administration of EBIO (800 nL of 0.4 mM) on non-convulsive seizure number within 1 hr of infusion. Data are from n = 4 DS mice. *p = 0.0189, two-tailed t test.

(C) Diagram of systemic administration of EBIO (25 mg/kg, subcutaneously [s.c.]).

(D) Quantification of effects of systemic administration of EBIO (25 mg/kg, s.c.) on non-convulsive seizure number within 30 min of EBIO infusion. Data are from n = 9 DS mice. **p = 0.0039 Wilcoxon signed-rank test.

(E) Representative ECoG and multiunit activity (MUA) traces from one DS mouse after vehicle and EBIO treatments.

(F) Magnification of the recordings indicated with red line in (E). Note the lack of seizures in ECoG and switch from bursting to single spike (arrows) firing mode after EBIO injection.