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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Cell Rep. 2019 Jan 2;26(1):54–64.e6. doi: 10.1016/j.celrep.2018.12.018

Figure 5. Optogenetic Disruption of Thalamic Bursting Aborts Non-convulsive Seizures in DS Mice.

Figure 5.

(A) Diagram showing that nRT modulates cortical rhythms through the relay thalamus.

(B) Diagram of optogenetic targeting of the thalamus. Inset: depth electrodes were positioned in VB thalamus.

(C) Top: example of optogenetic seizure interruption of an ongoing non-convulsive seizure in a DS mouse. The blue bar corresponds to a brief (50 ms) pulse of blue light to activate SSFO-expressing thalamocortical neurons. Unilateral activation of SSFO in the thalamus immediately interrupts the seizure. Bottom: spectrogram showing frequency components of the recorded seizure and decrease in power after SSFO activation.

(D) Magnification of the seizure interruption shown in (C). Activation of SSFO switches burst firing in the thalamus to tonic firing.

(E) Quantification of 1–25 Hz broadband power 2 s before and after optogenetic manipulation. Data are from 32 trials across 4 mice. ****p < 0.0001 Mann-Whitney test.