Figure 4. GABAergic Neurons Are Active at Different Levels During Pup Grooming vs. Infanticidal Behavior.
(A-B) Schematic of behavioral assay, injection strategy, and fiber implantation location for fiber photometry recording experiments.
(C) Representative injection site and fiber implantation for fiber photometry recording. Scale bar = 200 µm.
(D-F, H-I, K-L) Average Ca2+ signal changes during pup grooming in virgin females (D) and virgin males (E) and virgin males with infanticidal behavior (F), during pup crouching in virgin females (K) and virgin males (L), and during pup retrieval in virgin females (H) and virgin males (I). Mean ± SEM.
(G, J, M) Comparison of area under curve (AUC) per second during pup grooming and infanticidal behavior (G), pup retrieval (J), and pup crouching (M).
GCaMP6s virgin females, grooming, n = 16 trials; retrieval, n = 57; crouching, n = 40; GCaMP6s virgin males, grooming, n = 15; retrieval, n = 25; crouching, n = 35; infanticidal behavior, n = 7. EYFP control, grooming, n = 80; retrieval, n = 69; crouching, n = 204. (3~6 mice for each group). Mean ± SEM; one-way ANOVA with post-hoc Wilcoxon rank-sum with Bonferroni correction. n.s. not significant, *p < 0.05, ***p < 0.001.
See also Figure S3.