Figure 6. Cell Type Identities and Composition Do Not Differ Between Male and Female MeA.

(A-B) Schematic showing MeA dissection, single-cell dissociation, and sequencing using Drop-seq in virgin males and virgin females.

(C) Separation of 44,437 MeA cells from both males (21,715 cells) and females (22,722 cells) by principal component (PC) 1 and PC 2.

(D-F) Two-dimensional tSNE visualization showing the distribution of all cells (D), cells from males (E), and cells from females (F) among major MeA cell types.

(G-I) Two-dimensional tSNE visualization showing the distribution of all neurons (G), male neurons (H), and female neurons (I) among MeA neuronal subtypes. In (C-I), each dot corresponds to a single cell. Cells are colored according to major cell types (C, D) or neuronal subtypes (G). OPC, oligodendrocyte precursor cells; Olig., oligodendrocytes; OPC-OL, a transitional cell state between OPCs and oligodendrocytes.

(J) Bar plots showing the percentage of male and female cells in each neuronal subtype. There was no significant difference between males and females for any subtype (Wilcoxon rank-sum test, FDR > 0.05, n = 6 male samples (12 mice) and 7 female samples (20 mice; STAR Methods).

(K-L) Heat maps showing similar expression pattern of select top subtype markers across MeA neuronal subtypes between males (K) and females (L). The same set of markers are shown in K and L, and expression level is averaged within each subtype and normalized for each row (gene) (STAR Methods).

See also Figures S5, S6, and S7, Tables S1 and S2.