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. 2019 May 28;13(5):e0007450. doi: 10.1371/journal.pntd.0007450

Fig 6. Sera from hamsters vaccinated with rOv-TSPs block O. viverrini EV internalization by host cholangiocytes.

Fig 6

PKH67 labeled Ov-EVs was incubated with hamster antisera to rOv-TSP-2 or rOv-TSP-3 or rOv-TSP-2+rOv-TSP-3 or adjuvant or pre-immunization prior to co-culture with H69 cholangiocytes. Panel A = hamster pre-immunized serum, panel B = Adjuvant hamster serum, panel C = hamster antiserum to rOv-TSP-2, panel D = hamster antiserum to rOv-TSP-3, panel E = hamster antiserum to rOv-TSP-2 plus -3 and panel F = hamster antiserum to Ov-EVs. Panel G shows fluorescence intensity of internalization at 490 nm (green channel). The nuclei were stained by Hoechst (blue color) (**** P<0.0001).