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. 2019 May 22;15(5):e1008083. doi: 10.1371/journal.pgen.1008083

Fig 6. Focal adhesion localisation of the dPix-Git complex improves egg production and reduces morphogenetic defects.

Fig 6

(A) Schematic of dPix and Git transgenic rescue and structure function constructs with key domains indicated. Abbreviated annotations are: Coiled-coil (CC), Pleckstrin homology (PH), and Git binding domain (GBD). (B-C) Number of mature eggs produced per female for the indicated genotypes. Statistical tests are ANOVA, with post hoc Tukey’s test, and error bars are standard error of the mean. (B) Genotypes and sample sizes are: git/ Df(2R), n = 8; Git-GFP/ Df(2R), n = 29; ΔPBD-Git-GFP/ Df(2R), n = 24. (C) Genotypes and sample sizes are: dpix/ Df(2L), n = 7, dPix-GFP/ Df (2L), n = 7; rtGEF*dPix-GFP/ Df(2L), n = 13; ΔSH3-dPix-GFP/ Df(2L), n = 10. Significance: ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001; ns = not significant. (D-D’) Examples and frequencies of monolayered and multilayered follicular epithelia in ubi>Git-GFP (Wt in D’) and ubi>ΔPBDGit-GFP (ΔPBD in D’) rescue constructs. Scale bars are 20 μm. (D’) Stacked bar chart of frequencies in (D), statistical test is two-sided Fisher’s exact test. Significance: ** = p < 0.01. (E-E’) Examples and frequencies of normal and compound germline cysts in ubi>Git-GFP (Wt in E’) and ubi>ΔPBD-Git-GFP (ΔPBD in E’) rescue constructs. Scale bar is 20 μm. (E’) Stacked bar chart of frequencies in (E), statistical test is two-sided Fisher’s exact test. Significance: ** = p < 0.01. (F) Model for the basal signalling network controlling activation of myosin and monolayering during early egg chamber development. (G) Schematic of the effect of dpix, git mutation on epithelial organisation during egg chamber development. Abbreviated annotation: Extracellular matrix (ECM).