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. 2019 May 28;15(5):e1007829. doi: 10.1371/journal.ppat.1007829

Fig 4. Examination of RVFV CBD cap-binding.

Fig 4

A) Thermal stability of RVFV CBD and influenza A virus PB2 cap-binding domain was tested in presence and absence of different concentrations (1, 2, 5, 7.5, 10 mM) of m7GTP, m7GpppG, GTP and ATP. Melting temperatures are presented as mean and standard deviations of three independent measurements. Y-axis for RVFV CBD13 on the left (in black), y-axis for influenza A virus PB2 on the right (in grey). B) The affinity of RVFV CBD for m7GTP and GTP was measured by ITC at 25°C. RVFV CBD was in the cell at a concentration of 194 μM and m7GTP or GTP in the syringe at a concentration of 9.8 mM. The upper panel shows the raw data, the lower panel shows the integrated data fitted to a single-site binding model. The dissociation constant KD is given as a mean and standard deviation of three independent measurements for m7GTP.