Fig. 1.

Experimental strategy of gene deletion and gene rescue assays in mice. LSK cells were purified from poly I:C-treated mice. After culture in the presence of a cytokine cocktail (24 h), cells were transduced with retrovirus for expression of RhoA/GFP or GFP. Next day all the cells were transplanted to lethally irradiated congenital recipient mice. Sixteen weeks later, bone marrow and peripheral blood samples were analyzed by FACS using relevant antibodies to assess engraftment and blood cell differentiation