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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: J Microbiol Methods. 2018 Jul 11;152:135–142. doi: 10.1016/j.mimet.2018.07.005

Figure 1.

Figure 1.

A) Physical map of the IDTSMART-KAN_Std1_Xho1 recombinant plasmid construct with position and reverse orientation of Std1_Xho1 insert sequence indicated by solid, bold arrow and B) annotated Std1_Xho1 insert sequence. a Capital letters indicate inserted Xho1 restriction sites. x’s denote a proprietary sequence. Underlined bases denote EC23S587 assay target sequence for EPA Draft Method C. Italicized bases denote GenBac3 assay target sequence for EPA Method B. Underlined and italicized bases denote Entero1A assay target sequence for EPA Methods 1609.1 and 1611.1. Other bases correspond to the target sequence (not currently in use) for an additional assay.