Table 1. Vaccines and inflammatory agents.
The vaccines, TLR agonists, adjuvant and saline used in the present study are detailed. Vaccines were administered by injection of 1/10th the human dose in a 50 µL volume. TLR agonists, IFA and saline were also given in a single 50 µL volume. All injections were into the mouse hind leg quadriceps muscle.
| Vaccine/TLR | Components | Abbreviation | Manufacturer |
|---|---|---|---|
| Pentavac SD | Diphtheria, tetanus, pertussis (whole cell), hepatitis B (rDNA) and haemophilus type b conjugate vaccine | Pentavac | Serum Institute India |
| Agrippal | Trivalent flu subunits – H3N2, H1N1 and influenza B | Tri-Flu | Seqirus |
| Fluad | Trivalent flu subunits – H3N2, H1N1 and influenza B + MF59 | Tri-Flu + MF59 | Seqirus |
| Engerix B | Recombinant hepatitis B sAg absorbed on alum | GlaxoSmithKline | |
| IFA | Montanide ISA 51 VG | Seppic | |
| LPS | LPS-EB Ultrapure | Invivogen | |
| Poly I:C | Polyinosinic:polycytidylic acid | Sigma |