Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
y, w, Baz-GFP | Bloomington Drosophila Stock Center |
BDSC:51572; FLYB:FBtc0099880 |
FlyBase symbol: P{PTT-GC}baz CC01941 |
| Cell line (D. melanogaster) |
S2 | Invitrogen, Thermo Fisher Scientific |
Invitrogen:R69007 | |
| Antibody | Rabbit polyclonal anti-aPKC |
Santa Cruz | Santa_Cruz:sc-216 | IHC (1:1000) WB (1:3000) |
| Antibody | Mouse monoclonal anti-Par3 |
Ohshiro et al. (2000) | IHC (1:100) | |
| Antibody | Rabbit polyclonal anti-Par3 |
Ohshiro et al. (2000) | IHC (1:1000) WB (1:3000) |
|
| Antibody | Rabbit polyclonal anti-Par6 |
Izumi et al. (2004) | IHC (1:1000) | |
| Antibody | Chicken polyclonal anti-Myc |
Bethyl | Bethyl:A190-103A | IHC (1:1000) |
| Antibody | Mouse monoclonal anti-Miranda |
Ohshiro et al. (2000) | IHC (1:100) | |
| Antibody | Rabbit polyclonal anti-Lgl |
Ohshiro et al. (2000) | IHC (1:1000) WB (1:1000) |
|
| Antibody | Guinea pig polyclonal anti-Cdc42 |
Ulrich Tepass (University of Tronto) |
IHC (1:100) | |
| Antibody | Chicken polyclonal anti-GFP |
aves | aves:GFP-1020 | IHC (1:1000) |
| Antibody | Mouse monoclonal anti-GFP |
chemicon | chemicon:MAB3580 | IHC (1:1000) |
| Antibody | Rabbit polyclonal anti-GFP |
MBL | MBL:598 | IHC for Larva brain (1:1000) WB (1:2000) |
| Antibody | Mouse monoclonal anti-Flag |
Sigma | Sigma:F3165 | IHC (1:1000) |
| Antibody | Alexa 488, Cyanin3, Cyanin5 secondaries |
Jackson Immuno Research |
IHC for S2 cells (1:4000) IHC for Larva brain (1:200) |
|
| Antibody | anti-alpha tubulin | sigma | Sigma:T9026 | WB (1:15000) |
| Chemical compound, drug |
Latrunclin B | Wako | Wako:129–05101 | final concentration, 1 µM |
| Chemical compound, drug |
CuSO4 | Wako | Wako:034–04445 | final concentration, 1 mM |
| Recombinant DNA reagent |
pMT/V5-His B (Gateway vector) | Invitrogen, Thermo Fisher Scientific |
Invitrogen:V412020 | |
| Recombinant DNA reagent |
pAc5.1/V5-His B (Gateway vector) |
Invitrogen, Thermo Fisher Scientific |
Invitrogen:V411020 | |
| Recombinant DNA reagent |
pAc-Gal4 (Gateway vector) |
Addgene | Addgene:24344 | provided from Liqun Luo (Stanford University) |
| Recombinant DNA reagent |
pDAMCS (Gateway vector) |
this paper | Progenitors: pAct5C0 plasmids (BglII-XhoI fragment); Gateway vector pUC19 |
|
| Recombinant DNA reagent |
pUAST | Brand and Perrimon (1993) | ||
| Recombinant DNA reagent |
Par6-GFP (plasmid) | this paper | Progenitors: Par6 and GFP (cDNA); Gateway vector pAc5.1/V5-His B |
|
| Recombinant DNA reagent |
aPKC-GFP (plasmid) | this paper | Progenitors: aPKC and GFP (cDNA); Gateway vector pAc5.1/V5-His B |
|
| Recombinant DNA reagent |
myc-Par3-mKate2 (plasmid) | this paper | Progenitors: Par3 and mKate2 (cDNA); Gateway vector pMT/V5-His B, pAc-Gal4 or pDAMCS |
|
| Recombinant DNA reagent |
myc-Par3-GFP (plasmid) |
this paper | Progenitors: Par3 and GFP (cDNA); Gateway vector pMT/V5-His B, pAc-Gal4 or pDAMCS |
|
| Recombinant DNA reagent |
membrane-GFP | this paper | Progenitors: myristoylation tag from Fyn and GFP (cDNA); Gateway vector pAc5.1/V5-His B |
|
| Software, algorithm |
Huygens Professional |
Scientific Volume Imaging |
ver. 17.10 | |
| Other | DAPI | nacalai tesque | nacalai_tesque: 11034–56 |
IHC for S2 cells (1:4000) IHC for Larva brain (1:200) |