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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2018 Oct 5;139:29–35. doi: 10.1016/j.prostaglandins.2018.10.002

Figure 6. Effects of IsoLG modification on HDL functions.

Figure 6

HDL isolated by density gradient ultracentrifugation was modified with a range of IsoLG (0.1–3 molar equivalence to ApoAI) which produces adduct levels matching those found in patients with familial hypercholesterolemia or by myeloperoxidase oxidation, respectively. At the lower range of 0.1 eq, IsoLG-modified HDL is no longer protective against LPS-induced macrophage inflammation. Approaching 1 eq. modification, IsoLG-modified HDL contains crosslinked ApoAI which loses its ability to exchange and consequently has lower macrophage cholesterol efflux capacity. At greater than 1 eq., IsoLG-modification yields an HDL subpopulation with greater diameter.