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NAPE biosynthesis by pEcN or pNAPE-EcN in minimal media with different carbon sources. NAPEs were measured by LC/MS in bacteria grown overnight in LB, M9+glucose (M9g) or M9+aminoacids (M9a) media then induced in same media with IPTG for 18 h. A. Effect of media on total NAPE production by pEcN and pNAPE-EcN. B. Effect of media on profile of NAPE species produced by pNAPE-EcN.
