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Effect of incubation temperature on NAPE biosynthesis by pPLAAT2-EcN. pPLAAT2-EcN were grown in M9a media, induced with IPTG and then incubated overnight at either 37°C or 28°C. NAPEs were measured by LC/MS. A. Total NAPEs. B. Profile of NAPE species produced. * p<0.05 compared to 37°C.
