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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: ACS Infect Dis. 2017 Oct 17;4(1):3–13. doi: 10.1021/acsinfecdis.7b00127

Figure 6.

Figure 6.

Exogenous fatty acids alter the species of NAPE biosynthesized by pNAPE-EcN and pPLAAT2-EcN. A. Effect of Tween 80 (esterified C18:1n-9) on the amount of C18:1NAPE (as %total NAPEs) biosynthesized by pNAPE-EcN and pPLAAT2-EcN grown in M9a. B. Effect of Tween 80 on NAPE profile for pNAPE-EcN. * p<0.05 Sidak’s multiple comparison test for individual NAPE species. C. Effect of Tween 80 on NAPE profile for pPLAAT2-EcN. * p<0.05 Sidak’s multiple comparison test for individual NAPE species. D. Effects of stearic acid addition on % of total NAPE biosynthesized by pPLAAT2-EcN that are C18:0NAPE. E. Effects of oleic acid addition on % of total NAPE biosynthesized by pPLAAT2-EcN that are C18:1NAPE. F. Effects of adding a mixture of five free fatty acids (C18:0, C18:1, C18:2, C20:4, C22:6) on the profile of NAPE species biosynthesized by pPLAAT2-EcN The contribution of the three most prominent NAPE species produced in the absence of the fatty acid mixture (C16:1NAPE, C16:0NAPE, and C17cycNAPE) were summed for comparison.