Table 1:
Fungal DNA detection on NICU surfaces and in breastmilk.
| Sample type (number) | Sample number positive by qPCR1 (%) | Mean sequence reads/sample (range, number of samples sequenced)2 |
|---|---|---|
| Computer mouse (30) | 22 (73) | 1738 (range 166–6447, n=26) |
| Computer keyboard (30) | 21 (70) | 2986 (range 238–22075, n=22) |
| Incubator handles (31) | 21 (68) | 1938 (range 196–12858, n=28) |
| Stethoscope diaphragm (32) | 20 (61) | 1609 (range 268–5791, n=25) |
| Breastmilk (6) | 6 (100) | 11538 (range 320–34839, n=6) |
| “No template” control (20) | 2 (10) | 50 (range 1–143, n=4) |
| Breastmilk - no lysis control (12) | 0 (0) | NA |
1
Cq values indicated fungal DNA amounts above the limit of detection
2
NICU surfaces and breastmilk sequencing information is included only for samples that passed quality control as described in Materials and Methods