Fig. 2.

IGFBP7 exerts potent inhibitory effects on proliferation of ATC and FTC cell lines in vitro. a Western blotting assay validated the protein levels of IGFBP7 in ARO, FRO, and WRO cell lines overexpressed or silenced with IGFBP7. α-tubulin was used as a loading control. b–d Representative images and quantitation of IGFBP7-overexpressed or silenced cells at the indicated time points following initially plating the same number of cells. e MTT assay was conducted to measure the effect of IGFBP7 on the proliferation of the indicated cells at the indicated time points. f Representative micrographs (left) and relative quantification (right) of the indicated cells as evaluated by soft agar assay. All experiments were repeated three times with similar results. Data represent the mean ± S.D. of three independent experiments. A two-tailed Student’s t-test was used for statistical analysis (*P < 0.05, **P < 0.01)