Fig. 4.

IGFBP7 upregulates expression of p21^Cip1 and p27^Kip1 and induces G1-S arrest. a Western blotting analysis of levels of p21^Cip1, p27^Kip1, CDK2, cyclin E, phosphorylated Rb (p-Rb, Ser780) and total Rb in the indicated cells. α-tubulin was used as a loading control. b, c Representative micrographs (upper) and quantification of the proportions (lower) of BrdU incorporating cells in IGFBP7-overexpressed or-silenced cells, as well as corresponding vector-control cells. d Flow cytometric analysis of the indicated thyroid cancer cell lines with IGFBP7 overexpression or knock down. Data represent the mean ± S.D. of three independent experiments. * P < 0.05, ** P < 0.01