Table 1.

PCR and sequencing primers^a for bisulfite pyrosequencing

Region	Primer	Sequence (5′ to 3′)	CpGs	Chromosomal localization (bp)b	Amplicon size (bp)	Sequence to analyze
ICR1	Forward	TGGGTATTTTTGGAGGTTTTTTT	4	Chr11: 2,020,978 - 2,021,291	314	YGYGTYGTAGGGTTTAYGGGGGTT
	Reverse	cAACTTAAATCCCAAACCATAACA
	Sequencing	GTTTYGGGTTATTTAAGTTA
ICR2	Forward	TTGTTTATAAGGTGTAGATGGGAG	4	Chr11: 2,720,465 - 2,720,669	205	AYGTTTGTGATTTGGGAYGGTYGYGGGGTATATAGTTTATT
	Reverse	cTCTCCCAAACTCTCCTCAAC
	Sequencing	TAGGTTAGGTTGTATTGTTG

^aAdopted from Paganini et al., 2015 [12]

^bGenomic coordinates are based on Ensembl release 75

^cPrimer is biotinylated at the 5′ end