Figure 4.

Assessment of oxygenated phospholipid species in mouse liver. Typical MS spectra of phosphatidylethanolamine (PE) (A-a) and phosphatidylcholine (PC) (B-a) obtained from WT mouse liver. Analysis of oxygenated PE species (A-b) and oxygenated PC (B-b) in liver of PCBP1-deleted (KO) vs. wild type (WT) mice. Male mice were 5–6 weeks old and maintained on a synthetic, defined diet containing adequate, but not elevated, amounts of iron (50 ppm). Ferroptotic cell death signals (hydroperoxy-PE species) are shown as closed diamonds. N=6.