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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Mar 22;130:49–58. doi: 10.1016/j.yjmcc.2019.03.015

Figure 5. ADK disruption increases microtubule stabilization/detyrosination.

Figure 5

(A) Ventricular lysates were separated into different Free, Microtubule (MT), Membrane (Memb), and Cytoskeletal (CSK) fractions as depicted. (N is nucleus, G is golgi, SR is sarcoplasmic reticulum, TT is t-tubules, M is mitochondria, SL is sarcolemma, CSK is cytoskeleton, MT is microtubule, and sMT is stabilized microtubule). Cardiac lysates were analyzed by western blot for alpha tubulin (B–F) and detyrosinated tubulin (Glu-tubulin) (G–K). β-actin (L,M), desmin (L, N) and sarcomeric actin (L,O), were also measured in the triton insoluble cytoskeletal fraction. (n=4, 4, 5, and 6 for WT. cADK−/−, WT-TAC, and cADK−/− TAC respectively).