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. 2019 Jun 7;9:8354. doi: 10.1038/s41598-019-44590-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Diagram of sample collection procedure. (A) After slaughter, reproductive tracts were trimmed free of connective tissues; (B) The uterine horn ipsilateral to the corpus luteum was isolated. Starting from the utero-tubal junction (UTJ), locking tweezers were positioned every 8 cm to isolate the anterior, medial and posterior uterine thirds. (C) The ipsilateral anterior third was individually flushed by injecting 3 mL of PBS into the UTJ edge. (D) Intercaruncular endometrial samples were dissected from the UTJ (black arrow) and from the lengthwise intermediate region (white arrow) of the third, at the mesometrial side.