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. 2019 Jun 7;9:8354. doi: 10.1038/s41598-019-44590-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Summary and integration of the main results. Metabolomic measurements reveal an overall decrease in substrate concentration in uterine luminal fluid recovered from pregnant (Preg) compared to control cyclic (Con) cows on day 7 post oestrus. (1) Concentrations of two metabolites 12(S)-HETE and 15(S)-HETE, associated to the lipoxygenases pathway, were significantly greater in the Preg group. Transcripts for lipoxygenases were up- (ALOX12) and downregulated (ALOX15B) in the uterotubal junction of Preg animals, suggesting an endometrial origin of regulation of Lipoxygenases-derived metabolites in ULF. (2) Glycine and sarcosine were significantly lower in abundance in ULF recovered from Preg compared to Con cows. A downregulation of SLC6A9 (a Glycine transporter) transcripts was found in the Preg endometrial tissue, that was consistent with the lower Glycine concentration in ULF. Exposure to a day 7 embryo modulates the concentration of (3) phospholipids and (4) acylcarnitines concentration in ULF. We propose that metabolite composition of the ULF changes in response to a pre-hatching embryo in vivo.