Table 1.
Sums and ratios of amino acids (AA) and biogenic amines (BA) concentrations in uterine luminal fluid from Control (Con) and Pregnant (Preg) cows.
| Metabolite groups | Group | P valuea | Log2 Fold-changeb | |
|---|---|---|---|---|
| Con (n = 8) | Preg (n = 10) | |||
| Total AA | 19325.27 ± 1563.99 | 17447.25 ± 1895.85 | 0.36 | −0.15 |
| Non-essential AA | 18861.09 ± 1469.75 | 17136.99 ± 1859.45 | 0.37 | −0.14 |
| Acidic AA | 6766.67 ± 720.65 | 6154.43 ± 578.02 | 0.51 | −0.14 |
| Small neutral AA | 10951.75 ± 903.36 | 8130.94 ± 655.04 | 0.02 | −0.43 |
| Osmotic-stress protection AA | 11807.05 ± 940.68 | 8808.92 ± 710.92 | 0.02 | −0.42 |
| Glucogenic AA | 6538.66 ± 630.91 | 5201.54 ± 661.67 | 0.17 | −0.32 |
| Glutathione precursors AA | 11229.58 ± 997.77 | 9936.98 ± 996.13 | 0.39 | −0.18 |
| Total BA | 5342.97 ± 442.49 | 4989.55 ± 675.82 | 0.70 | −0.10 |
| Spermidine/Putrescinec | 0.18 ± 0.04 | 0.17 ± 0.02 | 0.83 | −0.09 |
| Spermine/Spermidined | 0.78 ± 0.16 | 0.57 ± 0.11 | 0.30 | −0.43 |
Values are expressed as nmol/cm2 of endometrial area; mean ± SEM. Metabolite groups definitions are in Supplemental Table S2. Groups of metabolites in bold were different between Con and Preg.
aStatistical analyses were carried out by one-way ANOVA.
bData are represented as fold-change of the metabolite concentration between Preg and Con groups.
cRatio of Spermidine to Putrescine was calculated to access the activity of Spermidine synthase.
dRatio of Spermine to Spermidine was calculated to access the activity of Spermine synthase.