Table 3.
Sums and ratios of Phospholipids concentrations in uterine luminal fluid from Control (Con) and Pregnant (Preg) cows.
| Metabolite groups | Group | P valuea | Log2 Fold-changeb | |
|---|---|---|---|---|
| Con (n = 8) | Preg (n = 10) | |||
| Total recoverable amounts of phospholipids | 478.90 ± 37.61 | 429.32 ± 20.76 | 0.23 | −0.15 |
| Total recoverable amounts of LysoPC | 297.98 ± 22.49 | 280.51 ± 13.05 | 0.49 | −0.09 |
| Total recoverable amounts of PC | 182.30 ± 16.40 | 148.81 ± 9.81 | 0.08 | −0.29 |
| Total LysoPC/Total PCc | 1.66 ± 0.13 | 1.92 ± 0.08 | 0.10 | 0.21 |
| Total PC aa | 108.42 ± 12.35 | 83.71 ± 7.22 | 0.08 | −0.38 |
| Total PC ae | 73.88 ± 5.56 | 65.10 ± 3.13 | 0.16 | −0.18 |
| Total MUFA (PC) | 41.44 ± 6.16 | 30.18 ± 3.57 | 0.11 | −0.45 |
| Total PUFA (PC) | 84.33 ± 7.74 | 67.26 ± 4.87 | 0.07 | −0.32 |
| Total SFA (PC) | 56.53 ± 4.45 | 51.37 ± 2.41 | 0.29 | −0.14 |
| MUFA (PC)/SFA (PC)d | 0.74 ± 0.10 | 0.59 ± 0.06 | 0.20 | −0.34 |
| PUFA (PC)/MUFA (PC)d | 2.19 ± 0.13 | 2.33 ± 0.12 | 0.45 | 0.08 |
| PUFA (PC)/SFA (PC)d | 1.42 ± 0.08 | 1.24 ± 0.03 | 0.04 | −0.20 |
Phospholipids were grouped in Phosphatidylcholines (PC) and Lysophosphatidylcholines (LysoPC), diacyl- (PC aa) or acyl-alkyl- (PC ae) phosphatidylcholines, saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) glycerophosphocholines. Values are expressed as nmol/cm2 of endometrial area; mean ± SEM. Metabolite groups definitions are in Supplemental Table S2.
Groups of metabolites in bold were different between Con and Preg group.
aStatistical analyses were carried out by one-way ANOVA.
bData are represented as fold-change of the metabolite concentration between Preg and Con groups.
cRatio of LysoPC to PC is an indicator of phospholipase activity.
dRatios of MUFA to SFA, PUFA to MUFA, and PUFA to SFA were measures of the activity of fatty acid desaturases.