Table 1.

The overall experimental design and results of the benchmarking study

Sample	# biological replicates	# technical replicates	Proteinase K concentration (μg/μl)	PIN	P-value
A	2	2	0.0200	0.645	3.17E-17
B	2	2	0.0100	0.714	2.07E-13
C	2	2	0.0040	0.850	2.73E-06
D	2	2	0.0020	0.868	2.15E-05
E	2	2	0.0010	0.900	9.87E-04
F	2	2	0.0005	0.933	0.039
Control	18	2	N.A.	0.964	0.721

P-values were obtained from the PIN algorithm by probability distribution fitting