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. 2019 Jun 7;9:8352. doi: 10.1038/s41598-019-44764-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Illustrations of sample mounting and final recording. (a1) An illustration of a prepared sample is presented: 1 metal frame, 2 fixating magnets, 3 petrolatum sealant, 4 glass coverslip, 5 collagen matrix, 6 PBS. (a2) Photography of a crafted mounting device with three mounting pots. (b) 3D visualization of a smaller section of an image cube of the TAMRA-labelled collagen scaffold is provided. The scale bar is 20 µm. (c) A half-width blend of the grey-value image of a collagen network surface height-map (left) using AFM and the resulting segmentation and detected pores (right) are presented. The scale bar is 20 µm. (d) Both methods provide almost identical results.