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. 2019 Jun 7;9:8352. doi: 10.1038/s41598-019-44764-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Crucial parameters with residual pore-size detection. (a) Visualization of detected 2D bubbles (circles) in an exemplary 2D image slice. Black depicts segmented collagen fibers, blue represents determined pores of a single analysis process, orange represents detected pores of a second residual analysis. The scale bar is 20 µm. (b) The histogram of detected pore diameters with median pore size $\tilde{ζ}$ _residual for the two collagen gels is shown. (c) The median per image-stack provides a robust measure of pore size ζ_residual. (d) The percentage of the fluid volume covered by the detected pore spheres (pore coverage) of 1.5 g/l matrices is increased compared to 3.0 g/l matrices. (e) The pseudo pore diameter measures a virtual pore diameter for completely spherical fluid volumes of both collagen matrices.