Figure 3.
The activation of Kv current and hyperpolarization induced by DEA‐NO is prevented by Kv7 channel inhibitors. (a and b) Mean values of Kv currents in PASMCs recorded after the application of depolarizing ramps from −60 to +20 mV in the presence of the Kv7 channel inhibitors (a) XE991 (10 μM, n = 6 from five animals) or (b) linopirdine (10 μM, n = 6 from five animals) before (black lines) and after (blue lines) the addition of DEA‐NO (10 μM). Inset shows the DEA‐sensitive current at −30 and +20 mV under control conditions (n = 10 from seven animals) and in the presence of XE991 or linopirdine (n = 6 from five animals). (c and d) Average values of DEA‐NO‐sensitive currents under both conditions obtained by subtracting the current in the presence and in the absence of DEA‐NO. (e and f) Mean values of the changes in the membrane potential induced by DEA‐NO in the absence (Ctrl) and in the presence of the Kv7 channel inhibitors (XE991, n = 6 from five animals, or linopirdine, n = 5). *P < 0.05 versus Ctrl (Student's unpaired t test). Results are means ± SEM