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. 2019 May 17;176(13):2209–2226. doi: 10.1111/bph.14667

Figure 4.

Figure 4

Apremilast regulated the leukocyte populations of mesenteric lymph nodes and lamina propria by suppressing the expression of chemokines and their receptors. (a) The percentage of macrophages (CD11b+F4/80+), neutrophils (CD11b+Gr‐1+), and dendritic cells (CD11b+CD11c+) in MLNs. (b) The percentage of naïve T cells (CD44−CD62L+, gated on CD3+CD4+), effector T cells (CD44+CD62L−, gated on CD3+CD4+), Th17 cells (CD4+IL‐17+, gated on CD3+), and Treg cells (CD25+Foxp3+, gated on CD3+CD4+) in MLNs. (c) The percentage of CD11b+ monocytes, macrophages, neutrophils, and dendritic cells in lamina propria. (d) The percentage of γδTCR+ T cells, CD4+ T cells, and CD8+ T cells in lamina propria. (e) Colonic tissues were immunofluorescence stained with CD11b, F4/80, and Ly6G, and the nuclei were stained with DAPI. (f) Colonic tissues were immunofluorescently stained for CXCR3 and CCR5. (g) The mRNA expression of chemokines and receptors in colon tissue. Data are shown as the representative images under flow cytometry and immunofluorescent staining. The summary data are shown as means ± SEM; n = 8 per group. *P < 0.05, significantly different from vehicle (DSS only) group