Figure 5.

Pharmacological inhibition of A_2A receptors prevents cerebral ischaemic injury. (a) TTC staining of coronal brain sections 24 hr after eMCAo and quantification of infarct size in vehicle‐ or KW 6002‐treated mice (n = 7 mice per group). (b) Neurological deficit scores of cerebral ischaemic vehicle‐ or KW 6002‐treated mice (n = 7 mice per group). (c) Real‐time PCR analysis of adhesion molecule expression in brains of vehicle‐ or KW 6002‐treated mice at 6 hr after eMCAo (n = 6 mice per group). (d) Real‐time PCR analysis of adhesion molecule expression in huBMECs treated with TNF‐α. huBMECs were pretreated with KW 6002 at 100 nmol·L⁻¹ for 30 min and then stimulated with 10 ng·ml⁻¹ TNF‐α for 2 hr (n = 6). (e) Western blot analysis and densitometric quantification of adhesion molecule expression in huBMECs treated with TNF‐α. huBMECs were pretreated with KW 6002 at 100 nmol·L⁻¹ for 30 min and then stimulated with TNF‐α at 10 ng·ml⁻¹ for 4 hr (n = 5). Data shown are means ± SD (for a–c) and means ± SEM (for d, e). *P < .05, significantly different as indicated; unpaired Student's t test (for a, c, d, and e) and Mann–Whitney U test (for b)