Fig. 2.

Phylogeny of Trypanosoma cruzi clade. Phylogenetic trees of Trypanosomacruzi clade inferred using combined gGAPDH and 18S rRNA gene sequences (1,363 bp). The phylogenetic trees with outgroup (Trypanosoma lewisi) were generated using a maximum likelihood method (A), neighbor joining method (B), and minimum evolution method (C). All bootstrap values from 1,000 replications are shown on the interior branch nodes. Our isolated Trypanosoma spp. are highlighted in bold and under line. GenBank accession numbers used in this phylogeny are listed in Table S1.