Table 3.
Results of the screening PCR compared to results for the 18S and cox1 gene sequencing results.
| Sample ID | Reference Sample ID | 18S rRNA Screening PCR (n = 43) |
18S rRNA sequence results (n = 17) |
cox1 sequencing results (n-40) |
|
|---|---|---|---|---|---|
| B. microti-like sp. | Babesia sensu stricto | Species (aSF, bND, c-) | Species (aSF, bND, c-) | ||
| GA-01 | Rac 2 | + | + | B. microti-like | – |
| GA-02 | Rac 008 | + | + | SF | Western group |
| GA-03 | Rac 122 | + | – | B. microti-like | Western group |
| GA-04 | Rac 123 | + | + | ND | B. microti-like |
| GA-05 | Rac 126 | + | + | SF | B. microti-like |
| GA-06 | Rac 169 | + | + | SF | B. microti-like |
| FL-01 | FL 1 | + | + | B. microti-like | – |
| FL-02 | FL 11 | + | – | ND | B. microti-like |
| FL-03 | FL 13 | + | + | ND | B. microti-like |
| FL-04 | FL 16 | + | – | ND | B. microti-like |
| FL-05 | FL 31 | + | + | SF | B. microti-like |
| FL-06 | FL 32 | + | – | ND | B. microti-like |
| WV-01 | WV 3 | + | – | ND | B. microti-like |
| WV-02 | WV 10 | – | + | Novel Babesia sp. | B. lotori-like |
| WV-03 | WV 14 | + | – | B. microti-like | B. microti-like |
| WV-04 | WV 16 | + | + | ND | B. lotori-like |
| PA-01 | PA-1 | + | – | B. microti-like | Novel Babesia sp. |
| PA-02 | PAF-6 | + | + | ND | B. lotori-like |
| PA-03 | PAF-9 | + | + | B. lotori-like | B. lotori-like |
| PA-04 | PAF-12 | + | + | ND | B. lotori-like |
| MN-01 | 15–7455 | + | – | B. microti-like | Novel Babesia sp. |
| MN-02 | 15–7986 | + | – | ND | B. microti-like |
| MN-03 | 15–11512 | + | – | SF | B. microti-like |
| MO-01 | MOI | + | + | ND | B. microti-like |
| MO-02 | Rac I | + | + | ND | B. microti-like |
| MO-03 | Rac L | + | – | B. microti-like | B. microti-like |
| MO-04 | Rac P | – | + | B. lotori-like | B. lotori-like |
| MO-05 | Rac Q | + | + | SF | B. lotori-like |
| TX-01 | TX 2 | + | + | ND | B. microti-like |
| ONT-01 | W1110-15 | + | + | ND | B. microti-like |
| ONT-02 | W504-15 | + | + | SF | B. microti-like |
| ONT-03 | W507-15 | + | + | Novel Babesia sp. | Novel Babesia sp |
| ID-01 | ID 2 | – | + | ND | B. lotori-like |
| CO-01 | CO 8 | + | + | Novel Babesia sp. | Novel Babesia spSF |
| CA-01 | SM 1 | + | + | SF | B. lotori-like |
| CA-02 | SM 2 | – | + | ND | B. lotori-like |
| CA-03 | SM 3 | + | – | B. microti-like | B. microti-like |
| CA-04 | Son 4 | – | + | B. lotori-like | B. lotori-like |
| CA-05 | SF 2 | + | – | ND | B. microti-like |
| CA-06 | CC3 | + | + | ND | B. microti-like |
| CA-07 | Mar 1 | + | – | B. microti-like | SF |
| CA-08 | Mar 3 | + | – | B. lotori-like | B. lotori-like |
| CA-09 | Mar 6 | + | + | SF | B. lotori-like |
a
SF, sequence failed (this sample was PCR positive but the sequence failed).
b
ND, not done (samples was not run for this PCR).
c
Sample negative for PCR.