Table 1.

Oligonucleotide sequences of primers and probes used for real-time PCRs and conventional PCRs in this study

Target	Name	Sequence (5′–3′) and probes	Source
P. humanus Cytochrome b	Duplex A–D	F: GATGTAAATAGAGGGTGGTT	[6, 12]
		R: GAAATTCCTGAAAATCAAAC
		FAM-CATTCTTGTCTACGTTCATATTTGG-TAMRA
		VIC-TATTCTTGTCTACGTTCATGTTTGA-TAMRA
	Duplex B–C/E	F: TTAGAGCGMTTRTTTACCC
		R: AYAAACACACAAAAMCTCCT
		FAM-GAGCTGGATAGTGATAAGGTTTAT-MGB
		VIC-CTTGCCGTTTATTTTGTTGGGGTTT-TAMRA
	Monoplex E	F: GGTTGGAATTGGATAGTGAT
		R: GGGTCCATAAAGAAATCCG
		FAM- TAGGAGGCTTTGTGTGTCTATCCT-TAMRA
	Cytb	F: GAGCGACTGTAATTACTAATC	[24]
		R: CAACAAAATTATCCGGGTCC
Acinetobacter spp. RNA polymerase β subunit gene	rpoB	F: TACTCATATACCGAAAAGAAACGG	[18]
		R: GGYTTACCAAGRCTATACTCAAC
		FAM-CGCGAAGATATCGGTCTSCAAGC-TAMRA
	rpoB (zone1)	F: TAYCGYAAAGAYTTGAAAGAAG	[25]
		R: CMACACCYTTGTTMCCRTGA
R. prowazekii rOmpB gene	ompB	F: AATGCTCTTGCAGCTGGTTCT
		R: TCGAGTGCTAATATTTTTGAAGCA
		FAM-CGGTGGTGTTAATGCTGCGTTACAACA-TAMRA
Y. pestis Plasminogen activator gene	PLA	F: ATGGAGCTTATACCGGAAAC	[26]
		R: GCGATACTGGCCTGCAAG
		FAM-TCCCGAAAGGAGTGCGGGTAATAGG-TAMRA
Borrelia spp. 16S ribosomal RNA	Bor16S	F: AGCCTTTAAAGCTTCGCTTGTAG	[27]
		R: GCCTCCCGTAGGAGTCTGG
		FAM-CCGGCCTGAGAGGGTGAACGG-TAMRA
B. quintana Hypothetical intracellular effector	yopP	F: TAAACCTCGGGGGAAGCAGA	[13]
		R: TTTCGTCCTCAACCCCATCA
		FAM-CGTTGCCGACAAGACGTCCTTG-TAMRA
3-oxoacyl-synthase gene	fabF3	F: GCGGCCTTGCTCTTGATGA
		R: GCTACTCTGCGTGCCTTGGA
		FAM-TGCAGCAGGTGGAGAGAACGTG-TAMRA
Anaplasma spp. 23S ribosomal RNA	TtAna	F: TGACAGCGTACCTTTTGCAT	[28]
		R: TGGAGGACCGAACCTGTTAC
		FAM-GGATTAGACCCGAAACCAAG-TAMRA
C. burnetii IS1111 spacer	IS1111	F: CAAGAAACGTATCGCTGTGGC	[29]
		R: CACAGAGCCACCGTATGAATC
		FAM-CCGAGTTCGAAACAATGAGGGCTG-TAMRA