Table 3.
Comparison of properties between C. lactoaceticus Xyn10B truncated variants and other thermophilic xylanases
| Xylanases from microorganism | Domaina | Mw (kDa)b |
Topt (°C) |
pHopt | Specific activity (IU/μmol) | References | |
|---|---|---|---|---|---|---|---|
| BWX | OSX | ||||||
| C. lactoaceticus Xyn10B-TM1 | A1-A2-A3-B | 91.0 | 65 | 6.0 | 944.3 ± 16.5 | 5371.5 ± 281.1 | This study |
| Xyn10B-TM2 | A3-B | 57.5 | 65 | 6.0 | 648.9 ± 10.3 | 3720.4 ± 217.8 | This study |
| Xyn10B-TM3 | A3-B-C1 | 73.6 | 65 | 6.5 | 457.5 ± 5.5 | 1106.1 ± 65.0 | This study |
| Xyn10B-TM4 | B | 39.3 | 65 | 6.5 | 284.0 ± 15.5 | 225.4 ± 31.8 | This study |
| Thermoanaerobacterium aotearoense SCUT27 XynAΔSLH | A1-A2-B-C1-C2 | 113 | 80 | 6.5 | 42,917.4 ± 361.6 | 24159.4 ± 180.8 | [20] |
| T. saccharolyticum NTOU1 XynA | A1-A2-B-C1-C2-D1-D2-D3 | 154 | 72 | 5.5 | 66836.0 ± 2464 | 22022.0 ± 1078.0 | [34] |
| Alkaline wastewater sludge Xyn-b39 | A1-A2-B-C1-D1-D2-D3 | 160 | 60 | 7.0 | 6588.8 ± 384 | 2464.0 ± 272 | [35] |
| Thermotoga thermarum Xyn10A | A1-A2-A3-B-C1-C2 | 131 | 95 | 7.0 | 19101.11 | NA | [36] |
| T. saccharolyticum B6A-RI XynA | A1-A2-B-C1-C2 | 130 | 70 | 5.5 | NA | 29562 | [37] |
| Bacillus sp. BP-23 xylanase C | A1-A2-B-C1-C2 | 120.6 | 45 | 5.0 | NA | 12.06 | [38] |
| T. neapolitana XynA | A1-A2-B-C1-C2 | 116 | 102 | 5.5 | NA | 12910.8 | [39] |
Xyn Xylanase, Mw molecular weight, Topt optimum temperature, pHopt optimum pH, BWX beech wood xylan, OSX oat spelt xylan, NA not available
aA1, A2, A3, B, C1, C2, D1, D2 represents the N and/or C-terminal domains, A1, A2, A3: N-terminal family 22 CBM; B: catalytic domain; C1, C2: C-terminal family 9 CBM; D1, D2, D3: C-terminal SLH
bValue by SDS-PAGE