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. Author manuscript; available in PMC: 2020 Jul 5.
Published in final edited form as: Biochem Biophys Res Commun. 2019 May 17;514(4):1204–1209. doi: 10.1016/j.bbrc.2019.05.098

Fig. 2. H2O2 treatment increases the number of ESA+/CD24−/CD44+ cells.

Fig. 2

Flow cytometry was employed to analyze the number of ESA+/CD24−/CD44+ BCSCs at 24 h after H2O2 treatment. (A) Representative flow cytometry graphs are presented, showing that H2O2 treatment increases the number of ESA+/CD24−/CD44+ cells. (B) Quantification of flow cytometry data demonstrates that H2O2 treatment increases the percentage of BCSCs in a dose-dependent manner. (C) A representative flow cytometry analysis of apoptosis in the ESA+/CD24−/CD44+ subpopulations. (D) Flow cytometry analysis revealed that H2O2 treatment has no significant effect on apoptotic cell death in BCSCs. Data are presented as mean ± SEM of three independent experiments. *** p< 0.001 vs. PBS control.