Table 1.
Gene names and real time fluorescent quantitative PCR primer used in this study
| Gene name | Protein name | Primer sequence (5′–3′) | Fragment length (bp) |
|---|---|---|---|
| CoEF1α | Elongation factor 1-alpha |
F:CATGATCACTGGTACCTCACAG R: CAAGGGTAAAGGCAAGCAAAG |
128.0 |
| KAR | 3-Ketoacyl-ACP-reductase |
F: TGCAAAAGCAGGAGTGATTG R: GGTGAAAACCTGTCCGGTAA |
83.4 |
| HAD | 3-Hydroxyacyl-ACP dehydrase |
F: CAGATTCCGGAAGCCAGTTA R: GGTTGCCTCAGGTTTTGCTA |
83.1 |
| EAR | Enoyl-ACP reductase |
F: TGGGCCAGAGGTCACTAAAC R: GCCTCAAAAGCAAGCACTTT |
83.7 |
| KASI | β-Ketoacyl-ACP-synthase I |
F: GCTCTGTCTCAGAGGAATGATG R: CTCCAGAACCTTCACCCATAAC |
91.0 |
| KASII | β-Ketoacyl-ACP-synthase II |
F: GGGAGAAGGAGCTGGAGTTT R: CTGGTGTGGATGTAGCATGG |
82.4 |
| SAD | Stearoyl-ACP-desaturase |
F: TTGGAGGATTGGGCTGAT R: AAGGGCTTCTTCTGTGAT |
198.0 |
| FATB | Palmitoyl-ACP thioesterase B |
F: AGTCCGATCAGCCCCTATCT R: GAGGATGATTCGCCATCACT |
84.6 |
| FAE1 | Fatty acid elongase 1 |
F: GACACTTCTGGTGTTCCTATCC R: TGACGAGCATCTTCTGGTTTAT |
99.0 |
| FAD2 | Fatty acid desaturase 2 |
F: CCCATTGTTTTCGTCGATCT R: TGTGTCATCAAGCCATTGGT |
84.3 |
| FAD3 | Fatty acid desaturase 3 |
F: GCCAAAAAAATCGGGTC R: ATCGCAAAGAATCACTCC |
83.0 |
| FAD7 | Fatty acid desaturase 7 |
F: ATTACCCGAACTCTGCG R: AGGACTTCTACCCCACA |
75.0 |
| FAD8 | Fatty acid desaturase 8 |
F: ATGACAAAGGACAGGCCAAC R: TCATGGCACCCATTATCTGA |
85.9 |
| GPD1 | Glycerol-3-phosphate dehydrogenase 1 |
F:ACGGGTTGGAAATGGGAAATA R:CCTTGACAGACGAGAACAGAAG |
99 |
| DGAT1 | Diacylglycerol acyltransferase 1 |
F:GACGATGTAACCGTTCTTGCG R:TTGGCTGCGGCTGCTGT |
178 |
| DGAT2 | Diacylglycerol acyltransferase 2 |
F:TGGAAGCCTGATGGGAAAC R:ATGCATTGGACGGTGATAGG |
111 |