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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: Nat Cell Biol. 2019 Apr 15;21(5):627–639. doi: 10.1038/s41556-019-0309-2

Figure 5. E-selectin ligands co-localize with Glg1 at the cell surface.

Figure 5

(a) Total internal reflectance fluorescence (TIRF) microscopy at the critical angle using a spinning disc was performed on SUM159-M1a cells probed with recombinant E-selectin-Fc (green) and anti-Glg1 (red). Scale bar represents 5 μm. (b) Confocal Z-slice of M1a cells probed with E-selectin-Fc (green), anti-Glg1 (red) and Hoechst (blue). Scale bar represents 5 μm. Data representative of 3 independent experiments (a, b).