Extended Data Fig. 5 |. PCDH1 ECl-specific mAb 3305 blocks ANDV and SNV entry into primary HPMECs.
HPMECs were preincubated with mAb 3305 or with human IgG control (Ctrl) (0–100 μg ml–1, 0–680 nM, in serial threefold dilutions), and then exposed to the indicated rVSVs Gn/Gc glycoproteins at a MOI of 0.2 IU per cell. Infected cells were scored at 9 hpi. One hundred per cent relative infectivity corresponds to 15–20% infected cells. Averages ± s.d.: three experiments; no-mAb samples (‘0’), n = 10 for ANDV and HTNV, n = 3 for SNV; mAb-treated samples, n = 5 for ANDV and HTNV, n = 3 for SNV. mAb 3305 versus control mAb, two-way ANO VA with Dunnett’s test: ns, P > 0.05; ****P < 0.0001.