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. 2019 Jun 8;18:105. doi: 10.1186/s12934-019-1130-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Diagram of the two E. coli expression constructs used in this study harbouring a the heterologous mevalonate pathway (MEV) leading towards the production of geraniol as expressed by pGER, and b an alcohol acyltransferase (AAT) capable of esterifying geraniol and acetyl-CoA to produce geranyl acetate as expressed by pET28a::RhAAT. Co-transformation of E. coli with the two plasmids, results in the production of geraniol and in vivo conversion to a geranyl acetate ester in high titres. The origin of all enzymes expressed on each plasmid is colour coded according to species of origin (inset)