Figure 3.

Analysis of the most stable reference gene by geNorm. (a) Stability ranking of RGs for normalization. M values were used to determine ranking of the stability of RGs where lower value indicates higher stability. (b) Optimal number of reference genes for normalization (NF_opt). The Pairwise variation (V value) was used to determine the optimal number of RGs where lower V value indicates optimal number of RGs for normalization. The value after V on the x-axis represents the number of RGs used. (c) Correlation between NF_opt and NF₃. Pearson's correlation was used to analyze correlation between NF_opt and NF₃. NF₃, the three most stable RGs for normalization. Group 1 or 2 or 3, the validation of the most stable RGs in largely expanded BM-MSCs or UCB-MSCs or BM- and UCB-MSCs, respectively.