Table 1.
Information of primers of candidate reference genes and their standard curve parameters.
| Information of primers | Standard curve parameters | ||||||
|---|---|---|---|---|---|---|---|
| Gene name (Symbol) | Sequence | Pro. size | Reference | R2 | M | B | E |
| 18S ribosomal RNA (18S) | F: CACGGACAGGATTGACAGATTG R: AACCAGACAAATCGCTCCAC |
92 | X03205.1 | 0.998 | -3.507 | 34.251 | 0.95 |
|
| |||||||
| Hypoxanthine phosphoribosyltransferase 1 (HPRT1) | F: CGAGATGTGATGAAGGAGATGG R: TGATGTAATCCAGCAGGTCAGC |
132 | NM_000194.2 | 0.999 | -3.499 | 32.221 | 1.01 |
|
| |||||||
| Beta-2-microglobulin (B2M) | F: CAGCGTACTCCAAAGATTCAGG R: GGATGAAACCCAGACACATAGC |
116 | NM_003406.3 | 0.995 | -3.452 | 37.651 | 1.01 |
|
| |||||||
| Eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) | F: ACTATCATTGATGCCCCAGGAC R: ACACCAGCAGCAACAATCAG |
121 | NM_001402.5 | 0.991 | -3.523 | 35.587 | 0.97 |
|
| |||||||
| Ribosomal protein, large, P0 (RPLP0) | F: TGGGCAAGAACACCATGATG R: CGGATATGAGGCAGCAGTTTC |
98 | NM_001002.3 | 0.998 | -3.511 | 37.016 | 0.96 |
|
| |||||||
| Peptidylprolyl isomerase A (PPIA) | F: TGCTGGACCCAACACAAATG R: AACACCACATGCTTGCCATC |
89 | NM_021130.3 | 0.990 | -3.388 | 33.650 | 1.03 |
|
| |||||||
| Actin. Beta (ACTB) | F: ACACCAGCAGCAACAATCAG R: CGGATATGAGGCAGCAGTTTC |
126 | NM_001002.3 | 0.997 | -3.447 | 36.891 | 0.99 |
|
| |||||||
| Tyrosine 3-monooxygenase/tryptophan 5- monooxygenase activation protein, zeta (YWHAZ) |
F: CGAAGCTGAAGCAGGAGAAG R: TTTGTGGGACAGCATGGATG |
111 | NM_003406.3 | 0.995 | -3.356 | 34.613 | 0.94 |
|
| |||||||
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F: ACGAAGCTGAAGCAGGAGAAG R: GGATGAAACCCAGACACATAGC |
105 | NM_002046.4 | 0.996 | -3.486 | 39.012 | 0.93 |
|
| |||||||
| TATA box binding protein (TBP) | F: AACCAGACAAATCGCTCCAC R: GGATGGGCATCATGGAAAC |
114 | NM_003406.3 | 0.996 | -3.374 | 33.282 | 1.03 |
|
| |||||||
| Octamer-binding transcription factor 4 (OCT4) | F: ACATCAAAGCTCTGCAGAAAGA R: AATACCTTCCCAAATAGAACCC |
127 | NM_002701 | - | - | - | - |
Pro. Size, product size (base pair); R2, correlation; M, slope; B, intercept; E, efficiency