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. 2019 May 22;2019:3231759. doi: 10.1155/2019/3231759

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Copyright © 2019 Luca Francetti et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MMP-1 and TIMP-1 expression. (a) Representative slot blot showing MMP-1 levels in serum-free cell culture supernatants of fibroblasts from healthy and overgrown gingivae. Immunoreactive bands were quantified by densitometric scanning as described in Materials and Methods. (b) Bar graphs showing MMP-1 protein levels analyzed by slot blot. Data are expressed as densitometric units and are mean ± SD for samples run in duplicate. (c) Bar graphs showing TIMP-1 gene expression after normalization on GAPDH mRNA levels. Data are expressed as mean ± SD for samples run in triplicate. ^∗p < 0.05 and ^∗∗p < 0.001.